| Round of 32 |
| Josh Ballard McKendree University | PIN | Jesse Francis Lindenwood-Belleville |
| Danny Apgar Northwest Missouri State Wrestling Club | PIN | Zach Long UCM |
| Jackson Morse ILL | PIN | Court Mace Lindsey Wilson College |
| Zachary Barkett University of the Cumberlands | TF 17-2 | Andrew Martin Ouachita Baptist University |
| Tyler Prazma MIZZ | TF 17-2 | Cody Willis McKendree University |
| Juan Stimpson Rend Lake College | DEC 3-1 | Luke Roth Lindenwood University |
| Clark Glass OKLA | MAJ DEC 8-0 | Brandon Lopez ILL |
| Taylor Doan Lindsey Wilson College | DEC 4-2 | Zachary King Lindenwood-Belleville |
| Gian Traverso Missouri Valley College | PIN | Lj Dowey Hannibal-LaGrange University |
| Walt Gillmor IOWA | MAJ DEC 10-1 | Samuel Mehan OKST |
| Tyler Caldwell OKST | PIN | Cadyn McKeeth Lindsey Wilson College |
| Darnell Thomas Lincoln College | TF 24-9 | Samuel Kolkow Wentworth Military Academy |
| Cody Pych Lindenwood University | TF 16-0 | Trever Dice Bethany College |
| Isaiah Gonzalez McKendree University | DEC 5-3 | Chase Espinosa MIZZ |
| Nicolas Haferkamp McKendree University | DEC 10-3 | Jordon Ward Bethany College |
| Zach Toal MIZZ | PIN | Justin Smith LIB |
