| Round of 32 |
| Dominique Bradley MIZZ | PIN | Tyler Smith Bethany College |
| Justin Smith Missouri Valley College | DEC 1-0 | Josh Carlyle Lindenwood University |
| De'von Thomas University of the Cumberlands | PIN | Jacob Clark UCM |
| Caleb King Benedictine College | DEC 4-1 | Darius Lewis Lindenwood-Belleville |
| Ross Janney McKendree University | DEC 6-3 | Morgan Denson Maryville University |
| Coltyn Pease Rend Lake College | PIN | Artie Bess IOWA |
| James Alter Missouri Valley College | DEC 3-1 | Ike Okenwa |
| Tyson Yoder OKST | PIN | Taylor Poe Ouachita Baptist University |
| Christopher Lopez ILL | TF 17-2 | Zach Schroeder McKendree University |
| Zach Weber McKendree University | PIN | Jamarcus McCardell Wentworth Military Academy |
| Austin Marsden OKST | DEC 12-6 | Mart Wilson LIB |
| Devin Mellon MIZZ | TF 18-3 | Martin Jones University of the Cumberlands |
| Jonathan Hupp Lindsey Wilson College | DEC 4-1 | Zack Wilcox Maryville University |
| Hunter Davis OKLA | PIN | Jose Camarillo |
| Donnell Walker Maryville University | DEC 7-2 | Matt Crawford Lindenwood University |
| Daniel Mueller Missouri Valley College | PIN | Tyler Cook Hannibal-LaGrange University |
