| Round of 64 |
| Ethan Raley Lincoln College | MAJ DEC 20-6 | Michael Anderson Kennedy King College |
| Lavion Mayes MIZZ | TF 17-0 | Tre Ponting Bethany College |
| Brandon Rice MIZZ | DEC 5-2 | Kyle Graumenz Neosho County Community College |
| Joseph Leach University of the Cumberlands | PIN | Ian Taylor Benedictine College |
| Keenan Hagerty Maryville University | DEC 5-2 | Michael Martin OKST |
| Logun Taylor SIUE | DEC 6-5 | Peter Crawford LIB |
| Tim Hamilton OKLA | MAJ DEC 12-3 | Connor Baxter OKST |
| Drake Houdashelt MIZZ | TF 16-1 | Joey Reis Lincoln College |
| Jordan Oliver OKST | PIN | Trent Robb Neosho County Community College |
| Courtney Strauss Bethany College | DEC 6-0 | Peter Tatanish LIB |
| Corey Duncan Lindenwood University | MAJ DEC 10-2 | Matthew Allibone Maryville University |
| Jake Ekster Missouri Valley College | MAJ DEC 18-10 | Tyler Sonnabend Hannibal-LaGrange University |
| Trevor Wiest MIZZ | DEC 8-2 | Devin Patterson McKendree University |
| Dexter Carter Ouachita Baptist University | PIN | Ty Ethridge Lindenwood-Belleville |
| Josh Kindig OKST | PIN | Aaron Engle UCM |
| Derrick Weller Lindenwood University | PIN | Javon Haines Neosho County Community College |
