| Round of 32 |
| James Krischke MIZZ | TF 17-2 | Michael Gregerson Maryville University |
| John Risewick Bethany College | TF 15-0 | Dalton Adams Missouri Valley College |
| Eliot Jurries Minnesota State | DEC 4-0 | Issac Podell LIB |
| Enoch McCottrell Lincoln College | MAJ DEC 17-5 | Daniel Romero Bethany College |
| Matthew Paxton Baker University | PIN | Keygan Foster Maryville University |
| Daniel Valles Lindenwood University | PIN | Rook Kassabaum Lincoln College |
| Michael Williams Northeastern Oklahoma A&M College | TF 20-4 | Kyle McClinton Hannibal-LaGrange University |
| Andrew Rettke McKendree University | DEC 9-6 | Paxon Fowler Baker University |
| Kevin Kinney Lindenwood University | TF 16-1 | Jeremiah Tucker-Shead Lincoln College |
| Alex Shea Neosho County Community College | DEC 5-2 | Paxton Edgar Lindenwood-Belleville |
| Daniel Rivera Missouri Valley College | PIN | Caleb Shuck Hannibal-LaGrange University |
| Tanner Mills Minnesota State | PIN | Marshall Phelan Labette Community College |
| Colton Orlando Lindenwood University | DEC 6-4 | Kyle Wilson Baker University |
| Tony Arellano Lindenwood University | TF 15-0 | Anthony Derrick Lincoln College |
| Cody Pae PUR | DEC 5-2 | Ryan Clevenger Northeastern Oklahoma A&M College |
| Trevor Feagans SIUE | TF 16-0 | Dylan Bethke Minnesota State |
