| Round of 32 |
| Dack Punke MIZZ | DEC 3-1 | Drew West NIU |
| Cameron Diep Lindenwood-Belleville | PIN | Andrew Rodriguez Lincoln College |
| Marshall Craig PUR | DEC 5-2 | Tanner Hitchcock Lindenwood University |
| Marco Padilla McKendree University | MAJ DEC 13-3 | Mike Aguirre Joliet Junior College |
| Jacob Seto Missouri Valley College | TF 20-4 | Joey Rivera Lincoln College |
| Jakob Allison ISU | TF 16-0 | Tre Ponce Labette Community College |
| Mikey Mascarenas Northeastern Oklahoma A&M College | DEC 9-8 | Tyler Kreith Maryville University |
| Dylan Pelland Baker University | MAJ DEC 11-2 | Jacob Bryson Joliet Junior College |
| Anthony Jagel Marian University (Ind.) | DEC 8-7 | Cameron Rudy Labette Community College |
| Carter Cox Ellsworth Community College | DEC 6-2 | Eric Arias Missouri Valley College |
| Dillon Prutch Labette Community College | PIN | Keegan Smith LIB |
| Jet Taylor OKST | DEC 10-5 | Camron Neal Ellsworth Community College |
| Majid Corbit Limestone University | DEC 7-3 | Christopher Sharp Marian University (Ind.) |
| Caleb Braley Davenport University | DEC 5-2 | Jonathan Sampson Cumberland University |
| Boo Dryden Northeastern Oklahoma A&M College | MAJ DEC 19-10 | Anthony Pisciotta Maryville University |
