| Round of 32 |
| Khanden Howse Ellsworth Community College | DEC 5-4 | Luke Ely LIB |
| Tanner Webster PUR | MAJ DEC 10-2 | Nicholas Whitehead Missouri Valley College |
| Adrian Cervantes Joliet Junior College | DEC 6-1 | Antonio Jenkins Lindenwood University |
| Brian Holloway Lincoln College | MAJ DEC 16-7 | Gavin Coleman Labette Community College |
| Sam Karel LIB | DEC 8-5 | Sam Greco Davenport University |
| Carlos Charley Diaz Northeastern Oklahoma A&M College | DEC 9-6 | Myron Crawford Ellsworth Community College |
| John Olivieri Cumberland University | DEC 9-3 | Ron Evans Lindenwood-Belleville |
| Glen Eisman Missouri Valley College | DEC 5-3 | Bryce Pilcher Ellsworth Community College |
| Jacob Mintzmyer Northeastern Oklahoma A&M College | PIN | Christian Johnson Labette Community College |
| Marcus Coleman ISU | TF 23-5 | Josh Culp Hannibal-LaGrange University |
| Devin Crawl Northeastern Oklahoma A&M College | DEC 8-1 | Christopher Cooper Davenport University |
| Zach Diviney Cumberland University | MAJ DEC 12-1 | Ryan Ragsdale Lindenwood-Belleville |
| Trenton Clines Drury University | DEC 4-3 | Michael Kent Ellsworth Community College |
| Cameron Simmons Marian University (Ind.) | DEC 4-3 | Dakota Shockley Missouri Valley College |
| Brit Wilson NIU | PIN | Corban Watson Hannibal-LaGrange University |
| Maxwell Southard ISU | PIN | Joe Brady UCM |
