| Round of 32 |
| Cevion Severado MIZZ | PIN | Matt Malavsky SIUE |
| Robert Rosser Missouri Valley College | DEC 5-3 | Adam Bender Kentucky Wesleyan College |
| Michael Crockett Ouachita Baptist University | DEC 10-4 | Nicholas Lindley Lindenwood-Belleville |
| Corey Cabanban ISU | DEC 3-1 | Mason Naifeh Northeastern Oklahoma A&M College |
| Nathan Cummings PUR | DEC 7-6 | Blake Bills Missouri Valley College |
| Carter Cox Ellsworth Community College | DEFAULT | Gabriel Sekou Lindenwood-Belleville |
| Hayden Williams Missouri Valley College | TF 17-0 | Thai Ta Ouachita Baptist University |
| Cameron Fusco McKendree University | MAJ DEC 9-1 | Gabe O'Callaghan Williams Baptist College |
| Matt Vinci NW | DEC 6-3 | Tre Ponce Labette Community College |
| Cameron Diep Lindenwood-Belleville | PIN | Barrett Stickelman Ouachita Baptist University |
| Robert Fairchild Williams Baptist College | DEC 10-6 | Jacob Roberson Labette Community College |
| Dillon Prutch Labette Community College | TF 17-2 | Michael (Dominic) Mota Lindenwood-Belleville |
| Joe Lupton Hannibal-LaGrange University | PIN | Dejay Nordrum Ellsworth Community College |
| Anthony Pisciotta Maryville University | TF 20-2 | Aaron Shaw Lincoln College |
| Montana Moon Oklahoma City | TF 18-3 | Jacob Dodd Lindsey Wilson College |
| Austin Plumlee Cumberland University | MAJ DEC 20-8 | Jeffri Juanes Northeastern Oklahoma A&M College |
