| Round of 32 |
| Nate Wishne William Jewell | DEC 9-6 | Ashton Massaro Triton College |
| Kason Mitchell Carl Albert State College | PIN | Jonny Marquez Indian Hills Community College |
| William Baysingar ILL | PIN | Dylan Reed Unattached |
| Nik Petsinger Rochester Community and Technical College | PIN | Wendel Hollister Marian University (Ind.) |
| Isaiah Perez Indian Hills Community College | DEC 1-0 | Blake Schmitt LIB |
| Jack Lage UCM | PIN | Collin Smith Cloud County Community College |
| Sabian Russell Quincy | PIN | Alexander Toro Wayland Baptist (Texas) |
| Sammy Shires Cumberland University | PIN | Conner Burrus University of the Cumberlands |
| Gunnar LeGrand Lindenwood University | TF 19-1 | Jesse Shannon Missouri Valley College |
| Kody Ketchum Maryville University | TF 18-2 | Trevon Swearinger Labette Community College |
| Aidan Medina Wayland Baptist (Texas) | PIN | Joseph Kenny Missouri Valley College |
| Hudson Goebel ILL | DEC 10-3 | Luke Kawa Morton College |
| Hayden Osment McKendree University | PIN | Victor Salazar Quincy |
| Kalvin LaPlant Labette Community College | MAJ DEC 11-3 | Mason McClung University of the Cumberlands |
| Dailey Jennings University of Central Oklahoma | DEC 8-5 | Blaine Ortiz Missouri Valley College |
| Ashton Hayhurst Triton College | PIN | Owen Manley Quincy |
| Paul Ishikawa Indian Hills Community College | MAJ DEC 17-6 | Jazen Brown Labette Community College |
| Jared Brown Marian University (Ind.) | PIN | Jacob Sinn Missouri Baptist University |
